Confirmation of introduced Agama picticauda in Florida based on molecular analyses
DOI:
https://doi.org/10.58782/flmnh.zkbc9978Keywords:
invasive species, Florida, native range origins, species identity, phylogenetics, AgamaAbstract
As of 2010, Florida had the largest number of introduced and established non-indigenous herpetofaunal species in the world. With the advent of molecular methodologies, researchers now are able to test hypotheses regarding introduction pathways, species identity, and native range origins. African Agamas, Agama Daudin 1802, found in Florida are hypothesized to be the African Rainbow Lizard, A. agama africana Hallowell 1844, based on color patterns and hypothesized native range origins (i.e., Benin, Ghana, and Togo) of imported specimens for the pet trade. However, recent systematic studies within the native range of the Agama complex have resulted in multiple taxonomic revisions, which calls into question the species identity of introduced populations in Florida. The purpose of this study is to determine the species identity of African agamas within Florida, as well as the native range origins of Florida populations. We conducted a comparative maximum likelihood analysis between individuals from Florida and individuals from the native range. Based on our results we determined that the species found in Florida is Agama picticauda from western Africa.
References
Campbell, T. S., S. Godley, R. Sandmann, and J. Horowitz. 2008. Geographic distribution: Agama agama africana (African Rainbow lizard). Herpetological Review 39:482.
Cassey, P. 2002. Life history and ecology influences establishment success of introduced land birds. Biological Journal of the Linnean Society 76:465-480. https://doi.org/10.1046/j.1095-8312.2002.00086.x
Connor, L. L., K. L. Krysko, T. Bourdreau, J.S. Roselius, and R.A. Coon. 2013. New county records for the Northern Curlytail Lizard, Leiocephalus carinatus Gray 1827 (Tropiduridae), and African Rainbow Lizard, Agama agama complex (Agamidae), in Florida. IRCF Reptiles and Amphibians 20:149-151. https://doi.org/10.17161/randa.v20i3.13956
Enge, K. M., K. L. Krysko, and B. L. Talley. 2004. Distribution and ecology of the introduced African Rainbow Lizard, Agama agama africana (Sauria: Agamidae), in Florida. Florida Scientist 67:303-310.
Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
Felsenstein, J. 2004. Inferring Phylogenies. Sinauer Associates, Sunderland, Massachusetts. 580 p.
Fujita, M. K., A. D. Leaché, F.T. Burbrink, J. A. McGuire, and C. Moritz. 2012. Coalescent-based species delimitation in an integrative taxonomy. Trends in Ecology and Evolution 27:480-488. https://doi.org/10.1016/j.tree.2012.04.012
Gouy, M., S. Guindon, and O. Gascuel. 2010. SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Molecular Biology and Evolution 27:221-224. https://doi.org/10.1093/molbev/msp259
Hamner, R. M., D. W. Freshwater, and P. E. Whitfield. 2007. Mitochondrial cytochrome B analysis reveals two invasive lionfish species with strong founder effects in the Western Atlantic. Journal of Fish Biology 71(Sb): 214-222. https://doi.org/10.1111/j.1095-8649.2007.01575.x
Hillis, D. M., and J. J. Bull. 1993. An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology 42:182-192. https://doi.org/10.1093/sysbio/42.2.182
Kearse, M., R. Moir, A. Wilson, S. Stones-Havas, M. Cheung, S. Sturrock, S. Buxton, A. Cooper, S. Markowitz, C. Duran, T. Thierer, B. Ashton, P. Mentjies, and A. Drummond. 2012. Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:1647-1649. https://doi.org/10.1093/bioinformatics/bts199
Kochen, T., W. Thomas, A. Meyer, S. Edwards, S. Paabo, F. Villablanca, and A. Wilson. 1989. Dynamics of mitochondrial DNA evolution in animals, amplification, and sequencing with conserved primers. Proceedings of the United States National Academy of Sciences 86:299-306. https://doi.org/10.1073/pnas.86.16.6196
Krysko, K. L., J. P. Burgess, M. R. Rochford, C. R. Gillette, D. Cueva, K. M. Enge, L. A. Somma, J. L. Stabile, D. C. Smith, J. A. Wasilewski, G. N. Kieckhefer, III, M. C. Granatosky, and S. V. Nielsen. 2011a. Verified non-indigenous amphibians and reptiles in Florida from 1863 through 2010: outlining the invasion process and identifying invasion pathways and stages Zootaxa 3028:1-68. https://doi.org/10.11646/zootaxa.3028.1.1
Krysko, K. L., K. M. Enge, and P. E. Moler. 2011b. Atlas of Amphibians and Reptiles in Florida. Final Report, Project Agreement 08013, Florida Fish and Wildlife Conservation Commission, Tallahassee.
Leaché, A. D., P. Wagner, C. W. Linkem, W. Böhme, T. J. Papenfuss, R. A. Chong, B. R. Lavin, A. M. Bauer, S. Nielsen, E. Greenbaum, M-O. Rödel, A. Schmitz, M. LeBreton, I. Ineich, L. Chirio, E. A. Eniang, S. Baha El Din, A. R. Lemmon, and F. T. Burbrink. 2014. A hybrid phylogenetic-phylogenomic approach for species tree estimation in African Agama lizards with applications to biogeography, character evolution, and diversification. Molecular Phylogenetics and Evolution 79:215-230. https://doi.org/10.1016/j.ympev.2014.06.013
Lockwood, J. L. 1999. Using taxonomy to predict success among introduced avifauna: relative importance of transport and establishment. Conservation Biology 13:560-567. https://doi.org/10.1046/j.1523-1739.1999.98155.x
Lockwood, J. L., D. Simberloff, M. L. McKinney, and B. Von Holle. 2001. How many, and which, plants will invade natural areas. Biological Invasions 3:1-8. https://doi.org/10.1023/A:1011412820174
Mediannikov, O., S. Trape, and J. F. Trape. 2012. A molecular study of the genus Agama (Squamata: Agamidae) in west Africa, with description of two new species and a review of the taxonomy, geographic distribution, and ecology of currently recognized species. Russian Journal of Herpetology 19:115-142.
Myers, R. L., and J. J. Ewel. 1990. Problems, prospects, and strategies for conservation. Pp. 619-632 in R. L. Myers, and J. J. Ewel, eds. Ecosystems of Florida. University of Central Florida Press, Orlando.
Saiki, R. K., D. H. Gelfand, S. Stoffel, S. J. Scharf, R. Higuchi, G. T. Horn, K. B. Mullis, and H. A. Erlich. 1988. Primer-directed enzymatic amplification of DNA with thermostable DNA polymerases. Science 239:487-491. https://doi.org/10.1126/science.2448875
Simberloff, D. 1997. The biology of invasions. Pp. 3-17 in D. Simberloff, D. C. Schmitz, and T. C. Brown, eds. Strangers in Paradise. Impact and Management of Non-indigenous Species in Florida. Island Press, Washington, D.C.
Tamura, K., G. Stecher, D. Peterson, A. Filipski, and S. Kumar. 2013. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution 30:2725-2729. https://doi.org/10.1093/molbev/mst197
Wagner, P., T. M. Wilms, A. Bauer, and W. Böhme. 2009. Studies on African Agama V. On the origin of Lacerta agama Linnaeus, 1758 (Squamata: Agamidae). Bonner zoologische Beiträge 56:215-223.
Wilson, L. D., and L. Porras. 1983. The ecological impact of man on the South Florida herpetofauna. University Kansas Museum of Natural History Special Publications 9:1-89. https://doi.org/10.5962/bhl.title.8435
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Bulletin of the Florida Museum of Natural History

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.